Protein_Domain
Part:BBa_K747070:Design
Designed by: Lucas Schneider Group: iGEM12_Freiburg (2012-09-25)
TAL-Protein_CG5_Direpeat
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
Source
This Part was synthesized and flanked with BsmBI-restriction sites.